1. Identifying and treating persons with Latent TB infection through targeted testing is an important goal of TB elimination because it can prevent the development of TB disease and stop further spread of TB to others.

2. Health care workers who serve patients and people who live or work in high-risk congregate settings are at higher risk for TB infection.

3. HIV patients, children under 5 years of age, people who are recently infected with M. Tuberculosis within the past 2 years and people who receive immunosuppressive therapy are at higher risk of developing TB disease.

4. After Mantoux Tuberculin Skin Test is administered, forearm should be examined within 48 to 72hrs by a trained health care worker, reaction is an area of induration or erythema that’s measured in millimeters.

5. When interpreting the TB skin test reaction, an induration of 5mm is considered as negative for recent contacts of people with infectious TB disease.

6. Infection with nontuberculous mycobacteria(NTM) or BCG vaccination can cause people to have a positive TB skin test reaction even if they do not have TB infection.

7. Anergy means absence of a reaction to skin test antigens because of immunosuppression. The absence of a reaction to tuberculin skin test does not rule out the diagnosis of TB infection or disease.

8. After TB germs have been transmitted to someone, it takes up to 4 weeks before TB infection can be detected by the tuberculin  skin test (TST) or an interferon-gamma release assay (IGRA).

9. IGRAs are preferred method of testing in groups of people who might be less likely to return for TB skin test reading and persons who are foreign born and received BCG vaccination.

10. Two-Step testing distinguishes between boosted reactions and reactions caused by recent infections. If initial skin test is negative, a second test should be given 1-3 weeks later.